تأثیر 8 هفته تمرین تناوبی خیلی شدید بر بیان ژن عوامل تنظیمی عضله زایی MyF5 و میوژنین در عضلۀ دوقلوی موشهای صحرایی | ||
| نشریه فیزیولوژی ورزش و فعالیت بدنی | ||
| مقاله 10، دوره 11، شماره 2 - شماره پیاپی 22، 1397، صفحه 109-116 اصل مقاله (1.54 M) | ||
| نوع مقاله: علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.48308/joeppa.2019.98928 | ||
| نویسندگان | ||
| سهیل بیگلری* ؛ عباسعلی گائینی | ||
| دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران | ||
| چکیده | ||
| هدف: باوجود نقش مهم فرایند عضله زایی در بازسازی و رشد عضلۀ اسکلتی، تأثیر تمرین های تناوبی خیلی شدید ) HIIT ( بر عوامل آن مشخص نیست. هدف از پژوهش حاضر، بررسی تأثیر هشت هفته HIIT بر عوامل مهم عضله زایی )عامل عضله زایی 5 ( MyF5 ) و میوژنین( در عضلۀ دوقلوی موش های صحرایی است. روش ها: در این مطالعه، 16 موش صحرایی نر نژاد ویستار با سن 10 هفته و محدودۀ وزنی 25 ± 225 گرم، به صورت تصادفی به دو گروه کنترل ) 8 = n ( و HIIT ( 8 = n ( تقسیم شدند. برنامۀ تمرین HIIT به مدت 40 دقیقه، سه روز در هفته و به مدت هشت هفته اجرا شد. 48 ساعت پس از آخرین جلسۀ تمرینی، عضلۀ دوقلو استخراج و میزان بیان ژن MyF5 و میوژنین به روش Real time-PCR سنجیده شد. به منظور تجزیه وتحلیل آماری داده ها، از آزمون t مستقل استفاده شد. نتایج: بیان ژن MyF5 و میوژنین در گروه HIIT در مقایسه با گروه کنترل افزایش معناداری ) 05 / 0p˂ ( داشت. همچنین، وزن عضلۀ دوقلو در گروه تمرینی در مقایسه با گروه کنترل به طور معنادار بالاتر بود ) 01 / 0p˂ .) نتیجه گیری: به نظر میرسد، HIIT منجر به افزایش بارز بیان ژن MyF5 ، میوژنین و وزن عضلۀ دوقلوی موش های صحرایی میشود. | ||
| کلیدواژه‌ها | ||
| عضله زایی؛ میوژنین؛ HIIT؛ MyF5؛ Real time-PCR | ||
| عنوان مقاله [English] | ||
| The effect of eight weeks of High-intensity Interval Training on the gene expression of the myogenic regulatory factors MyF5 and myogenin in the skeletal muscle of rats | ||
| نویسندگان [English] | ||
| Soheil Biglari؛ Abbas Ali Gaeini | ||
| Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran | ||
| چکیده [English] | ||
| Purpose: Myogenesis plays an important role in the skeletal muscle reconstruction and growth. However, the impact of High-intensity Interval Training (HIIT) on the factors of this process is unknown. The purpose of this study was to investigate the effect of 8 weeks HIIT on myogenesis main factors, i.e. Myogenic Factor 5 )Myf5( and Myogenin in the rat's gastrocnemius muscle. Methods: In this experimental study, sixteen male Wistar rats (at age of 10 weeks, weight= 255 ± 25 gr) were randomly divided into two groups (control (n = 8) and HIIT (n = 8)). HIIT protocol was performed for 40 minutes, three days a week for eight weeks. 48 hours after the last training session, gastrocnemius muscle was extracted and MyF5 and myogenin gene expression were measured by Real Time-PCR. To analyze the data, independent T-test was used. Results: Compared to the control group, MyF5 and myogenin gene expression significantly increased in the experimental group (P˂0.05). Additionally, gastrocnemius muscle weight in training group was significantly higher than the control group (P˂0.01). Conclusion: It seems that HIIT leads to a significant increase in the expression of MyF5, Myogenin, and gastrocnemius muscle weight in rats. | ||
| کلیدواژه‌ها [English] | ||
| HIIT, MyF5, Myogenesis, Myogenin, Real time-PCR | ||
| مراجع | ||
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1. Otto A, Collins-Hooper H, Patel A, Dash PR, Patel K. Adult skeletal muscle stem cell migration is mediated by a blebbing/ amoeboid mechanism. Rejuvenation research. 2011; 14(3): 249-260. 2. Zammit PS, Golding JP, Nagata Y, Hudon V, Partridge TA, Beauchamp JR. Muscle satellite cells adopt divergent fates. J Cell Biol. 2004; 166(3): 347-357. 3. Petrella JK, Kim J-s, Mayhew DL, Cross JM, Bamman MM. Potent myofiber hypertrophy during resistance training in humans is associated with satellite cell-mediated myonuclear addition: a cluster analysis. J Appl Physiol. 2008; 104(6): 1736-1742. 4. Martin NR, Lewis MP. Satellite cell activation and number following acute and chronic exercise: a mini review. Cell Mol Exerc Physiol. 2012; 1(1): e3. 5. Burd NA, West DW, Staples AW, Atherton PJ, Baker JM, Moore DR, Andrew MH. Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men. PloS one. 2010; 5(8): e12033. 6. Eksteen GJ. Satellite cell proliferation in response to a chronic laboratory-controlled uphill vs. downhill interval training intervention: Stellenbosch: University of Stellenbosch; 2006. Chapter 5, P. 88-96. 7. Smith HK, Maxwell L, Rodgers CD, McKee NH, Plyley MJ. Exercise-enhanced satellite cell proliferation and new myonuclear accretion in rat skeletal muscle. J Appl Physiol. 2001; 90(4): 1407-1414. 8. Matsakas A, Friedel A, Hertrampf T, Diel P. Short‐term endurance training results in a muscle‐specific decrease of myostatin mRNA content in the rat. Acta Physiologica. 2005; 183(3): 299-307. 9. Coffey VG, Hawley JA. The molecular bases of training adaptation. Sports Med. 2007; 37(9): 737-763. 10. Baar K. Training for endurance and strength: lessons from cell signaling. Med Sci Sports Exerc. 2006; 38(11): 1939-1944. 11. Gibala MJ, McGee SL. Metabolic adaptations to short-term high-intensity interval training: a little pain for a lot of gain? Exerc Sport Sci Rev. 2008; 36(2): 58-63. 12. ESTES RR, MALINOWSKI A, PIACENTINI M, THRUSH D, Salley E, Losey C, Hayes, E. The Effect of High Intensity Interval Run Training on Cross-sectional Area of the Vastus Lateralis in Untrained College Students. IJES. 2017; 10(1): 137. 13. Robinson MM, Dasari S, Konopka AR, Johnson ML, Manjunatha S, Esponda RR, Rickey E. Carter, Ian R. Lanza, and K. Sreekumaran Nair. Enhanced protein translation underlies improved metabolic and physical adaptations to different exercise training modes in young and old humans. Cell Metab. 2017; 25(3): 581-592. 14. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007; 14(6): 753-760. 15. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate‑intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep. 2015; 12(2): 2374-2382. 16. Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, Ljubkovic M. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013: cvt080. 17. Wisløff U, Loennechen JP, Falck G, Beisvag V, Currie S, Smith G, Ellingsen Ø. Increased contractility and calcium sensitivity in cardiac myocytes isolated from endurance trained rats. Cardiovasc Res. 2001; 50(3): 495-508. 18. Bazgir B, Fathi R, Valojerdi MR, Mozdziak P, Asgari A. Satellite cells contribution to exercise mediated muscle hypertrophy and repair. Cell Journal (Yakhteh). 2017; 18(4): 473-484. 19. Nederveen J, Joanisse S, Séguin C, Bell K, Baker S, Phillips S, et al. The effect of exercise mode on the acute response of satellite cells in old men. Acta Physiologica. 2015; 215(4): 177-190. 20. McCroskery S, Thomas M, Maxwell L, Sharma M, Kambadur R. Myostatin negatively regulates satellite cell activation and self-renewal. J. Cell Biol. 2003; 162(6): 1135-1147. 21. Roostaei M, gaeine A, Kordi MR. The difference of myostatin gene expression in fast and slow twitch healthy male rat after eight weeks of high intensity interval training. Physiology of Exercise and Physical Activity. 2016; 9(1): 10.[in Persian]. 22. Walsh F, Celeste A. Myostatin: a modulator of skeletal-muscle stem cells. Portland Press Limited; 2005. 23. Martins C, Kazakova I, Ludviksen M, Mehus I, Wisloff U, Kulseng B, et al. High-intensity interval training and isocaloric moderate-intensity continuous training result in similar improvements in body composition and fitness in obese individuals. Int J Sport Nutr Exerc Metab. 2016; 26(3): 197-204. 24. Bell KE, Séguin C, Parise G, Baker SK, Phillips SM. Day-to-day changes in muscle protein synthesis in recovery from resistance, aerobic, and high-intensity interval exercise in older men. J. Gerontol: Series A. 2015; 70(8): 1024-1029. | ||
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